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2024, Vol: 1, Issue: 4
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Original Article
Online Published: 09 Nov 2024
 


Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.

Farzana Afroz, hasnaif Mohammad Shifat, mst. Deloara Begum, mir Rowshan Akter, md. Mominul Islam.


Abstract
Aims: Extended-spectrum beta-lactam (ESBL) antibiotics resistance is one of the top antimicrobial threats globally. Recent evidence suggests the emergence of ESBL-positive Escherichia coli from the animal origin foods. However, information on the animal origin ESBL-positive E. coli is very limited in Bangladesh. The present study investigated the frequency and characteristics of ESBL-positive E. coli in milk samples from cows with mastitis in northern Bangladesh.
Methods: The phenotype of ESBL-positive E. coli was determined using ESBL-CHROM agar media and the antibiotic sensitivity was measured by disk diffusion method. PCR was used to detect the E. coli and ESBL-genes of the isolates.
Results: This study detected 60 E. coli isolates from 400 milk samples. Among the isolates, ESBL-positive E. coli was detected in 14 (23.33%) isolates according to the results of phenotypic confirmatory methods. Our findings further demonstrated that the most common ESBL gene was blaCTX-M (92.86%), subsequently 71.43% of the isolates had blaTEM. Most of the isolates bearing blaCTX-M gene also harbored with blaTEM gene (78.57%). Interestingly, blaSHV gene was absent in all isolated E. coli strains. Antibiogram revealed 100% of ESBL-positive isolates showed multidrug-resistant (MDR) in nature and exhibited complete resistance to ampicillin. Additionally, 92.86% were resistant to cefuroxime, cefaclor, and cefotaxime, while 85.72% showed resistance to cefpodoxime, amoxicillin-clavulanic acid, and aztreonam. Notably, all isolates were 100% sensitive to carbapenems.
Conclusion: This study provides noble evidence on the animal borne ESBL producing E. coli and raises need for vigilant antibiotic stewardship and robust infection control measures.

Key words: Escherichia coli, mastitis, antibiotic resistance, multidrug resistance


 
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How to Cite this Article
Pubmed Style

Afroz F, Shifat hM, Begum mD, Akter mR, Islam mM, . Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. Eu J Microbiol Infect Dis. Online First: 09 Nov, 2024. doi:10.5455/EJMID.20241024053102

Web Style

Afroz F, Shifat hM, Begum mD, Akter mR, Islam mM, . Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. https://www.wisdomgale.com/ejmid/?mno=225891 [Access: November 10, 2024]. doi:10.5455/EJMID.20241024053102

AMA (American Medical Association) Style

Afroz F, Shifat hM, Begum mD, Akter mR, Islam mM, . Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. Eu J Microbiol Infect Dis. Online First: 09 Nov, 2024. doi:10.5455/EJMID.20241024053102


Vancouver/ICMJE Style

Afroz F, Shifat hM, Begum mD, Akter mR, Islam mM, . Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. Eu J Microbiol Infect Dis, [cited November 10, 2024]; Online First: 09 Nov, 2024. doi:10.5455/EJMID.20241024053102


Harvard Style

Afroz, F., Shifat, h. M., Begum, m. D., Akter, m. R., Islam, m. M. & (0) Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. Eu J Microbiol Infect Dis, Online First: 09 Nov, 2024. doi:10.5455/EJMID.20241024053102


Turabian Style

Afroz, Farzana, hasnaif Mohammad Shifat, mst. Deloara Begum, mir Rowshan Akter, md. Mominul Islam, and . 0. Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. European Journal of Microbiology and Infectious Diseases, Online First: 09 Nov, 2024. doi:10.5455/EJMID.20241024053102


Chicago Style

Afroz, Farzana, hasnaif Mohammad Shifat, mst. Deloara Begum, mir Rowshan Akter, md. Mominul Islam, and . "Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.." European Journal of Microbiology and Infectious Diseases Online First: 09 Nov, 2024. doi:10.5455/EJMID.20241024053102


MLA (The Modern Language Association) Style

Afroz, Farzana, hasnaif Mohammad Shifat, mst. Deloara Begum, mir Rowshan Akter, md. Mominul Islam, and . "Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.." European Journal of Microbiology and Infectious Diseases Online First: 09 Nov, 2024. Web. 10 Nov 2024 doi:10.5455/EJMID.20241024053102


APA (American Psychological Association) Style

Afroz, F., Shifat, h. M., Begum, m. D., Akter, m. R., Islam, m. M. & (0) Molecular characterization of extended spectrum β-lactamase producing Escherichia coli isolated from bovine mastitic milk in Northern Bangladesh.. European Journal of Microbiology and Infectious Diseases, Online First: 09 Nov, 2024. doi:10.5455/EJMID.20241024053102


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